Objective To develop and initially validate a humanistic care experience scale in the stroke unit, and to provide a reliable tool to evaluate the quality of humanistic care in the stroke unit. Methods The scale was developed through literature review and two rounds of Delphi expert consultation.Then a convenience sample of 661 patients in the stroke unit were recruited across 70 hospitals in 20 provinces between June and August 2025 to validate the scale. Results The final scale consisted of 32 items across 4 dimensions (admission experience, core hospitalization care and response, empowerment and cooperation caring, discharge planning and evaluation).Exploratory factor analysis extracted 4 factors, explaining 69.536% of the variance.Confirmatory factor analysis demonstrated a good fit （χ2/df=2.112,RMSEA=0.071,CFI=0.912,IFI=0.904,TLI=0.904,NFI=0.886）.The item-level content validity index ranged from 0.917 to 1.000, and the scale-level content validity index using the average approach was 0.996.The Cronbach′s α coefficient for the total scale was 0.973, and the test-retest reliability was 0.925. Conclusion The huma-nistic care experience scale for patients in the stroke unit demonstrates satisfactory reliability and validity.