Objective To investigate the current status and influencing factors of artificial intelligence (AI) anxiety among undergra-duate nursing students, and to provide a reference for nursing education. Methods A convenience sample of 321 undergraduate nur-sing students were selected from 5 universities in Henan Province to fill out the Artificial Intelligence Anxiety Scale, the Attitude toward the Nursing AI Questionnaire, and the General Self-Efficacy Scale. Results The average AI anxiety score among undergra-duate nursing students was （65.50±10.95）.The degree of mastery of AI technology, attitude towards AI, self-efficacy, and students′ academic years were factors affecting AI anxiety （all P＜0.05）. Conclusion The level of AI anxiety among undergraduate nursing students is moderate.It is suggested that nursing educators strengthen AI related training, reshape students′ attitudes toward AI, and enhance students′ self-efficacy, in an effort to mitigate their AI anxiety