Objective To integrate the best evidence of cue-based oral feeding management for premature infants and carry out evidence-based practice (EBP) so as to improve the efficiency of oral feeding in premature infants. Methods The best evidence was obtained through literature retrieval. An EBP program for oral feeding management of premature infants was constructed based on baseline audit and barriers analysis. The implementation rate of audit criteria by nursing staff and the efficiency of oral feeding in premature infants before (June-November 2024) and after (February-July 2025) roll-out of the EBP program. Results After roll-out of the EBP program, the efficiency of oral feeding in premature infants was significantly improved, and the time to achieve full oral feeding was significantly shortened (both P<0.05); the implementation rate of each audit criterion for oral feeding management of premature infants by nursing staff was significantly increased (all P<0.05). Conclusion Carrying out the evidence-based cue-based oral feeding management program can standardize the practice of nursing staff in oral feeding of premature infants and play a positive role in improving oral feeding competence of premature infants.