Objective To observe the clinical efficacy of Huo-Long cupping therapy as an adjuvant intervention for preventing deep vein thrombosis (DVT) in patients with gynecological malignancies undergoing laparoscopic surgery. Methods A total of 76 patients scheduled for laparoscopic surgery for gynecological malignancies were randomly divided into a control group and an intervention group, with 38 cases in each group. The control group received routine perioperative nursing and standard prophylaxis for venous thromboembolism, while the intervention group additionally received Huo-Long cupping therapy on the day before surgery and 1, 3, and 5 days after surgery. The incidence of DVT, D dimer levels, hemodynamic indicators, and comfort level were compared between the two groups before and after the intervention. Results After the intervention, the incidence of DVT and D dimer level in the intervention group were significantly lower than those of the control group, while the blood flow velocities of the left popliteal vein and left common femoral vein, the General Comfort Questionnaire score in the intervention group were significantly higher than those of the control group (all P＜0.05). Conclusion As an adjuvant therapy, Huo-Long cupping therapy can effectively reduce the risk of postoperative DVT in patients with gynecological malignancies undergoing laparoscopic surgery, suppress excessive elevation of D dimer, improve lower extremity venous blood flow, and enhance patient comfort.