Objective To investigate and analyze the clinical practice training needs of respiratory specialist nurses, thereby providing evidence for developing a competency-based training program that is oriented toward nurses′ needs. Methods A preliminary framework of clinical practice training content for respiratory specialist nurses was constructed based on systematic literature review and existing training programs, which as followed by a two-rounds Delphi expert consultation to refine and form a training framework comprising 8 dimensions with 27 items. Subsequently, a Kano model questionnaire was designed based on the framework, which was utilized to conduct a nationwide survey among 195 conveniently selected respiratory specialist nurses. Results Among the 27 clinical practice training needs, 6(22.2%) were classified as must-be quality needs, 12(44.4%) as one-dimensional quality needs, 5(18.6%) as attractive quality needs, and 4(14.8%) as indifferent quality needs. Conclusion Clinical practice training needs in respiratory specialist nurses demonstrates obvious multi-level and diversity. Training institutions should develop tailored training programs based on these distinct need attributes to enhance training quality and nurse satisfaction.