Objective To evaluate the effectiveness of a self-disclosure-themed humanistic care program based on Watson′s Theory of Human Caring in the standardized training of newly recruited nurses. Methods Using a convenience sampling method, 80 newly recruited nurses were enrolled and assigned to either an intervention group (n=40) or a control group (n=40) according to hospital campus. The control group received the routine standardized training program, whereas the intervention group additionally participated in a self-disclosure-themed humanistic care program developed based on Watson′s Theory of Human Caring. The training program lasted for 6 months. After the intervention, humanistic caring attributes, self-disclosure, job satisfaction, departmental competency assessment scores, and humanistic caring competency were evaluated and compared between the two groups. Results After the 6-month standardized training program, the intervention group achieved significantly higher scores for self-disclosure, humanistic caring attributes, job satisfaction, and humanistic caring competency in the departmental competency assessment than the control group (all P<0.05). No statistically significant difference was found between the two groups in case-based assessment scores (P>0.05). Conclusion The self-disclosure-themed humanistic care program based on Watson′s Theory of Human Caring effectively enhances self-disclosure, humanistic caring competency, and humanistic caring attributes among newly recruited nurses while improving job satisfaction. This training approach may facilitate the development of humanistic nursing practice among newly recruited nurses.