Objective To develop a multidimensional assessment-based exercise intervention program for patients with chronic insomnia, and to evaluate its effectiveness in improving sleep quality. Methods An exercise intervention program for patients with chronic insomnia was developed based on literature review and expert consensus meeting.A total of 58 patients with chronic insomnia were recruited using convenience sampling, then they were divided into an intervention group and a control group according to their wil-lingness to participate in exercise intervention.The control group received routine nursing care, while the intervention group additionally received the exercise intervention program.Twelve weeks after the intervention, sleep quality and emotional outcomes were compared between the two groups. Results The exercise intervention program consisted of four domains (multidisciplinary management, pre-exercise assessment, exercise principles, and exercise modality and dose) and 22 items.After the intervention, the scores of sleep quality, anxiety and depression in the intervention group were significantly lower than those in the control group (all P＜0.05). Conclusion The developed exercise intervention program could improve sleep quality and alleviate anxiety and depressive symptoms in patients with chronic insomnia.